Our Ractopamine (RAC) Rapid Test Kit is an advanced fluorescence immunochromatographic assay engineered for quantitative detection of ractopamine residues in feed, meat, and animal urine samples.Powered by highly specific antibodies and cutting-edge fluorescence signaling, the kit delivers reliable results in 10-15 minutes, with a detection range of 0.5-200 ng/mL—fully aligned with international regulations
The Ractopamine Rapid Test Kit is a fluorescent immunochromatographic assay used for quantitative analysis of Rac in feed, tissues, and urine
Ractopamine has a strong and long-lasting effect on relaxing bronchial smooth muscle and is used as an anti-asthma drug in humans. After entering the animal body, it can change the metabolic pathway of nutrients, promote animal muscle growth, accelerate the conversion and decomposition of fat, and increase the lean meat rate of livestock. It is one of the β-agonist illegally produced in animal husbandry. Eating food with excessive Ractopamine residues can cause human poisoning, so most countries prohibit its use in livestock and poultry breeding. Ractopamine residues in food can be determined by methods such as HPLC or GC-MS. However, these traditional methods usually require very expensive preparation procedures.
Compared with traditional methods,the xinyu Ractopamine Rapid Test Kit is simple, fast, and sensitive, and is suitable for the detection of large quantities of samples.
| Manufacturer | Shandong Xinyu Biotechnology Co., LTD |
|---|---|
| Trademark | XinyuBio |
| Production lot number | FRAC（FA）240724 |
| Analyte | Ractopamine |
| Sample type | feed, tissue, and urine. |
| Method | Fluorescence quantitative immunochromatography |
| Package | 40T |
| Limit of detection | 0.2 ppb |
| Storage | Keep away from light and dry and store it at a temperature ranging from 4 to 28℃. It is recommended to refrigerate it at 4℃ in the refrigerator. Do not freeze it. |
| Shelf life | The package is valid for 6 months |
The unique 3-card joint detection design enables the sample detection quantity and detection items to be independently selected and matched: multiple items and multiple specimens are detected simultaneously.
Easy to learn and ready to use right away. The pretreatment process is unified, and a single sample extraction can meet the needs of multiple tests.
High - repeatability sample detection. Average recovery rate over 90%, high sensitivity and good repeatability.
Paired with our fully automatic integrated incubator and fluorescence reader, this solution eliminates the cumbersome process of establishing standard curves for users. Users can effortlessly obtain accurate quantitative results in only 7 minutes.
Test strips: 5×8 strips (40 strips total)
Microwells (Reaction wells): 5×8 wells (40 wells total)
Sample Diluting Buffer: 1 bottles (20ml/bottle)
1 IC card for XinyuBio Fluorescence immunoanalyzer
Balance (accuracy: 0.01g)
Centrifuge
Pulverizer
Oscillator
50ml centrifugal tube or conical bottle
10~100μL、50-1000 μL pipette and pipette tips
Fully automatic incubator
XinyuBio Fluorescence immunoanalyzer
Add 100 μl diluent
Weigh 5g samples through 20 mesh screens
Add 25 ml of extract
Shake for 1 minute and let it sit for 5 minutes
Centrifuge at 4000 RPM for 5 minutes
Take the supernatant and dilute and mix well
Centrifuge at 4000 RPM for 5 minutes
Take 100 μL of supernatant and add it into micropores
Incubate for 7 minutes
Read and print
The manufacturer will provide you with free calibration products for experimental calibration. The operation method can be directly started from the dilution step. Please calibrate the equipment with the supporting reagents before the experiment, so that you will get more satisfactory results every time. At least one calibration is required for each reagent kit, and one calibration every two weeks. In case of any abnormality, please contact the manufacturer.
1、Turn on the multifunctional incubator to preheat at least 30 minutes in advance.
2、Take a representative sample of not less than 1kg, crush it, sift it through a 20-mesh sieve, and mix it evenly.
3、Prepare 50% ethanol (extract): 1∶1 (v/v) ethanol/water.
4、 Put the micro-wells into the micro-well carrier of the incubator.
Pipette 100ul of the sample diluent and add it to the micro-wells.
Insert the test strip of the corresponding item and place it on the carrier.
Press the "Start Key” at the lower right corner of the device to start the program.
Termination: Add 100 µL of the termination solution to each hole, and read the absorbance value at 450/630nm double wavelengths immediately.
Weigh 5.00g of the sample and add it to 40ml of the extract. Vortex the mixture for 1 minute using a vortex mixer, and then let it stand for more than 1 minute (the standing is to facilitate the aspiration of the supernatant after extraction. If the sample amount is small, centrifugation or filtration can be used to accelerate this process. Samples such as wheat and barley need centrifugation, not standing).
Add 400 μL of the sample dilution solution to the centrifuge tube, and then pipette 100 μL of the supernatant from the standing sample extract into the centrifuge tube. After thorough shaking, centrifuge at 7000 rpm for 1 minute (or at 4000rmp for 5 minutes).
After the alarm notification sound ends (the time can be extended),
Press the Start Key, and the lower tray will eject automatically.
Pipette 100μl supernatant after dilution and centrifugation and add it to the microwells.
Press the "Start Key” and the device will run automatically.
The countdown will be displayed on the device, and the device will give an alarm 15s in advance before the end of the experiment.
Read the IC card: Place the IC card in the card reading area at the upper right - hand corner of the reader, and click the "Scan" button to enter the data. (This step can be operated in advance, and it is sufficient to scan once for each box.)
Insert the triple test strip holder into the card slot, select the corresponding strip holder channel, choose the "" button in the "Detection" drop - down menu, and click the "Read Value" button. The reader will automatically insert the card and read the value. (XinyuBio reader has functions such as one - click data import to a USB flash drive, viewing historical data, printing data, and quality control comparison. You can contact the manufacturer for specific usage.)
| Name | XinyuBio Fluorescence Immunoanalyzer |
|---|---|
| Trademark | XinyuBio |
| Model number | iTest Q300 |
| Test item | Mycotoxins, Veterinary drug residues, Pesticide residues |
| Input power | 60VA |
| Rated current | ≤5A |
| Measuring channel | 3 |
| Display | Touch screen display |
| Printer | Built-in microprinter |
| Port | USB, RS232, network and other interfaces |
| Name | XinyuBio Full automatic Incubator |
|---|---|
| Trademark | XinyuBio |
| Model number | iTest Q300 |
| Channels | 8*3 |
| Temperature control range | Room temperature ~65℃ |
| Time setting | 0~100h |
| Temperature control accuracy | ≤0.5℃ |
| Display resolution | ±0.1℃ |
| Temperature uniformity | ≤0.5℃ |
| Amplitude of oscillation | Amplitude 2mm |
| Oscillation mode | Reciprocating vibration |
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