1. Hazards of Deoxynivalenol (DON/Vomitoxin)

• Severe Gastrointestinal Irritation: Swine are highly susceptible; low doses induce vomiting, feed refusal, diarrhea, and gastrointestinal mucosal inflammation.

• Immunotoxicity: Impairs intestinal barrier function and suppresses immune cell activity, reducing disease resistance in livestock.

• Growth & Reproductive Toxicity: Causes poor feed conversion, stunted growth, and reproductive issues including abortion and weak offspring in breeding livestock.

• Food Safety Risk: Thermostable and resistant to common food processing; accumulates in the food chain, causing nausea, vomiting, dizziness, and fatigue in humans.

• 
  

2. EU & US Regulatory Limits

EU Standards (Regulation (EU) 2023/915, effective July 1, 2024)

• Unprocessed cereals (excluding oats): 1,000 μg/kg (1 ppm)

• Unprocessed durum wheat & corn: 1,500 μg/kg (1.5 ppm)

• Unprocessed oats (with husk): 1,750 μg/kg (1.75 ppm)

• Cereal milling products & pasta: 600 μg/kg (0.6 ppm)

• Bakery & breakfast cereals: 400 μg/kg (0.4 ppm)

• Infant/young children’s cereal foods: 150 μg/kg (0.15 ppm)

US FDA Advisory Levels

• Finished wheat products (flour, bran, germ) for human consumption: 1,000 μg/kg (1 ppm)

• Grains for beef/poultry (>4 months): 10 ppm (88% dry matter)

• Grains for swine: 5 ppm (max 20% of diet)

• Distillers/brewers grains for ruminants: 30 ppm (88% dry matter)

• 

3. Industries & Companies Requiring DON Testing

• Grain traders, elevators, and storage facilities

• Feed mills & premix plants (raw material & finished product QC)

• Livestock farms (swine, poultry, cattle operations)

• Food processors: flour, baking, brewing, pasta, breakfast cereals

• Export/import traders (EU/US market compliance)

• Third-party labs & agricultural regulatory agencies

4. DON Testing Methods

• Fluorescent Quantitative Rapid Test Strips: 8–10 min, portable quantitative, ideal for on-site screening

• ELISA Quantitative Kits: Microplate-based, high-throughput lab quantitation

• Immunomagnetic Bead Purification Kits: Clean-up for HPLC/LC-MS, high recovery & accuracy

• LC-MS/GC-MS: Official reference method, confirmatory testing

• 
  

5. Our Company’s DON Testing Products

DON ELISA Quantitative Test Kit

• Principle: Competitive ELISA

• Samples: Feed, grains, cereals

• Range: 250–5,000 ppb

• Time: ~20 mins

• Features: Stable, high-throughput lab quantitation

• Storage: 2–8°C, 12-month shelf life

• 
  

DON Fluorescent Quantitative Test Strip

• Principle: Quantum dot fluorescent immunochromatography

• Samples: Corn, wheat, grains

• Range: 50–6,000 ppb; LOD: 50 ppb

• Time: 8–10 mins

• Features: Rapid on-site quantitative, portable

• Storage: 4–30°C dry, 6-month shelf life

• 
  

DON Immunomagnetic Bead Purification Kit

• Principle: Specific immunoaffinity beads

• Samples: Grains, oils, cereals

• Recovery: ≥85%

• Use: For HPLC/LC-MS clean-up

• Storage: 2–8°C, 12-month shelf life