Complete Guide to Zearalenone Detection

Zearalenone (ZEN) is a naturally occurring mycotoxin that is mainly found in cereal grains and their products. It has strong estrogenic activity and can cause serious damage to the reproductive system of animals, while also posing potential risks to human health through the food chain. This article details the hazards of zearalenone, relevant national standards, applicable enterprises, detection methods, and introduces the corresponding detection products of Shandong Xinyubio Biotechnology Co., Ltd., providing a complete solution for zearalenone detection and compliance control.

1. Hazards of Zearalenone

• It has strong estrogenic activity, which can interfere with the endocrine system of animals, leading to abnormal reproductive development, such as pseudopregnancy, abortion, and reduced reproductive capacity.

• It can damage the intestinal barrier function, reduce the body's immunity, and affect the normal growth and development of animals, resulting in economic losses.

• It is heat-resistant and not easy to be decomposed by conventional processing methods. It can enter the human body through the food chain, posing hidden dangers to human reproductive health and endocrine balance.

2. National Standards and Limitations

Food Field (GB 2761-2017)

Feed Field (GB 13078-2017)

• Raw materials and compound feeds: The content of zearalenone shall not exceed 50 μg/kg.

• Corn, wheat and other grain raw materials: The content of zearalenone shall not exceed 100 μg/kg.

  
  

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3. Enterprises That Need Detection

• Grain processing enterprises, grain storage and trading enterprises (for raw material quality control).

• Feed production enterprises (for raw material acceptance and finished product quality control).

• Aquaculture enterprises, animal husbandry farms (for feed quality inspection).

• Food processing enterprises (for grain-based raw material detection).

• Third-party testing institutions and market supervision departments (for compliance inspection and supervision).

4. Detection Methods

• High Performance Liquid Chromatography (HPLC): As the standard arbitration method, it has high accuracy and is suitable for precise quantitative detection.

• Enzyme-Linked Immunosorbent Assay (ELISA): It is the preferred method for daily batch detection, with simple operation and high efficiency.

• Immunochromatography: Suitable for on-site rapid screening, with fast detection speed and simple operation.

• Magnetic bead purification: Used for sample pretreatment to improve detection accuracy and reduce matrix interference.

  
  

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5. Shandong Xinyubio Biotechnology Co., Ltd. Products

5.1 ELISA Kit

• Detection Principle: Competitive ELISA, with pre-coated antigen on the detection plate, and quantitative detection through standard curve.

• Technical Parameters: Detection range covers 0-1000 ppb, reaction time is short, and it is suitable for batch quantitative detection.

• Application Scenario: Daily quality control of feed and food enterprises, with simple operation and stable data.

  
  

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5.2 Fluorescent Quantitative Rapid Detection Card

• Detection Principle: Based on immunochromatography technology, it can quickly detect zearalenone content, with a detection limit of 1 μg/kg.

• Advantages: Fast detection (within 10 minutes), no need for professional equipment, suitable for on-site rapid screening.

  
  

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5.3 Magnetic Bead Purification Kit

• Purification Principle: Specific adsorption of zearalenone through specific antibodies, removing matrix interference.

• Application: Cooperate with HPLC for accurate detection, suitable for arbitration and re-inspection scenarios.

6. Application Guidelines

• For on-site rapid screening: Use fluorescent quantitative detection cards for quick judgment.

• For laboratory accurate detection: Use ELISA kits for batch quantitative detection.

• For arbitration and re-inspection: Use magnetic bead purification kits combined with HPLC for precise detection.