Ochratoxin Quantitative Rapid Test Kit

ochratoxin（OTA） rapid test kit

ochratoxin rapid test kit is a lateral flow immunochromatographic assay that determines a qualitative level for the presence of ochratoxin and is intended for use in grains, feed materials and feed products. This method is suitable for the rapid on-site test.
Ochratoxin presents risks to food safety similar to other mycotoxins. When ingested through contaminated food, this toxin represents a risk to animal and human health. It is immunosuppressive, mutagenic, carcinogenic, and cytotoxic. As a result, various industries worldwide are increasing regulations to protect consumers from the harmful effects of ochratoxin intake.
For instance, the European Union established guidance levels for feed and maximum levels for food, and China instituted permissible ochratoxin levels in feed. Other countries such as Indonesia, Singapore, Korea, and Malaysia have also established regulations.

ochratoxin rapid test kit Operation guidance

calibration

The manufacturer will provide you with free calibration products for experimental calibration. The operation method can be directly started from the dilution step. Please calibrate the equipment with the supporting reagents before the experiment, so that you will get more satisfactory results every time. At least one calibration is required for each reagent kit, and one calibration every two weeks. In case of any abnormality, please contact the manufacturer.

preparatory work

1、Turn on the multifunctional incubator to preheat at least 30 minutes in advance.

2、Take a representative sample of not less than 1kg, crush it, sift it through a 20-mesh sieve, and mix it evenly.

3、Prepare 50% ethanol (extract): 1∶1 (v/v) ethanol/water.

4、Put the microporous strip into the micropores of the incubator carrier, pipette 100 μl of the sample dilution solution into the micropores, insert the test strip of the corresponding item and place it on the carrier for later use.

Termination: Add 100 µL of the termination solution to each hole, and read the absorbance value at 450/630nm double wavelengths immediately.

extractive

Weigh 5.00g of the sample and add it to 40ml of the extract. Vortex the mixture for 1 minute using a vortex mixer, and then let it stand for more than 1 minute (the standing is to facilitate the aspiration of the supernatant after extraction. If the sample amount is small, centrifugation or filtration can be used to accelerate this process. Samples such as wheat and barley need centrifugation, not standing).

attenuation

Add 400 μL of the sample dilution solution to the centrifuge tube, and then pipette 100 μL of the supernatant from the standing sample extract into the centrifuge tube. After thorough shaking, centrifuge at 7000 rpm for 1 minute (or at 4000 rpm for 3 minutes).

load sample

Pipette 100 μl of the supernatant after centrifugation into the micropore and blow it for 10 seconds to mix it thoroughly.

incubation

Close the carrier on the incubator, let the test card fall, take out the test card after accurately timing for 7 minutes, and insert it into the reader to read the result within 1 minute.

interpretation of result

Read the IC card: Place the IC card in the card reading area in the upper right corner of the reader, and click the scanning button to enter the data (this step can be operated in advance, and scanning once per box is sufficient).

Push the triple test card into the slot, select the corresponding card holder channel, click the "DON" button in the "Detection" drop-down menu, and click the "Start Detection" button. The reader will automatically read the card value. (The Longke New Domain reader has the functions of one-click data import to a USB drive, viewing historical data, printing data, quality control comparison, etc. For specific use, you can contact us.)