T-2 toxin Rapid Test Kit Operation guidance
calibration
The manufacturer will provide you with free calibration products for experimental calibration. The operation method can be directly started from the dilution step. Please calibrate the equipment with the supporting reagents before the experiment, so that you will get more satisfactory results every time. At least one calibration is required for each reagent kit, and one calibration every two weeks. In case of any abnormality, please contact the manufacturer.
preparatory work
1、Turn on the multifunctional incubator to preheat at least 30 minutes in advance.
2、Take a representative sample of not less than 1kg, crush it, sift it through a 20-mesh sieve, and mix it evenly.
3、Prepare 50% ethanol (extract): 1∶1 (v/v) ethanol/water.
4、Put the microporous strip into the micropores of the incubator carrier, pipette 100 μl of the sample dilution solution into the micropores, insert the test strip of the corresponding item and place it on the carrier for later use.
Termination: Add 100 µL of the termination solution to each hole, and read the absorbance value at 450/630nm double wavelengths immediately.
extractive
Weigh 5.00g of the sample and add it to 40ml of the extract. Vortex the mixture for 1 minute using a vortex mixer, and then let it stand for more than 1 minute (the standing is to facilitate the aspiration of the supernatant after extraction. If the sample amount is small, centrifugation or filtration can be used to accelerate this process. Samples such as wheat and barley need centrifugation, not standing).
attenuation
Add 400 μL of the sample dilution solution to the centrifuge tube, and then pipette 100 μL of the supernatant from the standing sample extract into the centrifuge tube. After thorough shaking, centrifuge at 7000 rpm for 1 minute (or at 4000 rpm for 3 minutes).
load sample
Pipette 100 μl of the supernatant after centrifugation into the micropore and blow it for 10 seconds to mix it thoroughly.
incubation
Close the carrier on the incubator, let the test card fall, take out the test card after accurately timing for 7 minutes, and insert it into the reader to read the result within 1 minute.
interpretation of result
Read the IC card: Place the IC card in the card reading area in the upper right corner of the reader, and click the scanning button to enter the data (this step can be operated in advance, and scanning once per box is sufficient).
Push the triple test card into the slot, select the corresponding card holder channel, click the "DON" button in the "Detection" drop-down menu, and click the "Start Detection" button. The reader will automatically read the card value. (The Longke New Domain reader has the functions of one-click data import to a USB drive, viewing historical data, printing data, quality control comparison, etc. For specific use, you can contact us.)
