① Preparation
Add 100 μl diluent
② Weighing
Weigh 5g samples through 20 mesh screens
③ Extraction
Add 25 ml of extract
④ Vibration
Shake for 1 minute and let it sit for 5 minutes
⑤ Centrifugation
Centrifuge at 4000 RPM for 5 minutes
⑥ Dilution
Take the supernatant and dilute and mix well
⑦ Centrifugation
Centrifuge at 4000 RPM for 5 minutes
⑧ Sample loading
Take 100 μL of supernatant and add it into micropores
⑨ Incubation
Incubate for 7 minutes
⑩ Reading
Read and print
Zearalenone(ZON/ZEN) Rapid Test Kit Operation guidance
Calibration
The manufacturer will provide you with free calibration products for experimental calibration. The operation method
can be directly started from the dilution step. Please calibrate the equipment with the supporting reagents before the
experiment, so that you will get more satisfactory results every time. At least one calibration is required for each
reagent kit, and one calibration every two weeks. In case of any abnormality, please contact the manufacturer.
Preparatory work
1、Turn on the multifunctional incubator to preheat at least 30 minutes in advance.
2、Take a representative sample of not less than 1kg, crush it, sift it through a 20-mesh sieve, and mix it evenly.
3、Prepare 50% ethanol (extract): 1∶1 (v/v) ethanol/water.
4、 Put the micro-wells into the micro-well carrier of the incubator.
Pipette 100ul of the sample diluent and add it to the micro-wells.
Insert the test strip of the corresponding item and place it on the carrier.
Press the "Start Key” at the lower right corner of the device to start the program.
Termination: Add 100 µL of the termination solution to each hole, and read the absorbance value at 450/630nm double
wavelengths immediately.
Extractive
Weigh 5.00g of the sample and add it to 40ml of the extract. Vortex the mixture for 1 minute using a vortex mixer, and
then let it stand for more than 1 minute (the standing is to facilitate the aspiration of the supernatant after
extraction. If the sample amount is small, centrifugation or filtration can be used to accelerate this process.
Samples such as wheat and barley need centrifugation, not standing).
Dilution
Add 400 μL of the sample dilution solution to the centrifuge tube, and then pipette 100 μL of the supernatant from the
standing sample extract into the centrifuge tube. After thorough shaking, centrifuge at 7000 rpm for 1 minute (or at 4000rmp for 5 minutes).
Sample loading
After the alarm notification sound ends (the time can be extended),
Press the Start Key, and the lower tray will eject automatically.
Pipette 100μl supernatant after dilution and centrifugation and add it to the microwells.
Incubation
Press the "Start Key” and the device will run automatically.
The countdown will be displayed on the device, and the device will give an alarm 15s in advance before the end of the experiment.
Reading
Read the IC card: Place the IC card in the card reading area at the upper right - hand corner of the reader, and click the "Scan" button to enter the data. (This step can be operated in advance, and it is sufficient to scan once for each box.)
Insert the triple test strip holder into the card slot, select the corresponding strip holder channel, choose the "ZON" button in the "Detection" drop - down menu, and click the "Read Value" button. The reader will automatically insert the card and read the value. (XinyuBio reader has functions such as one - click data import to a USB flash drive, viewing historical data, printing data, and quality control comparison. You can contact the manufacturer for specific usage.)
