Professional Zearalenone Immunoaffinity Purification Kit using Magnetic Bead Method for accurate detection of ZEN mycotoxin in cereals, grains and oils. High recovery rate ≥85%.
(Magnetic Beads Method)
High-performance purification solution for accurate detection of zearalenone (ZEN) mycotoxin in cereals, grains and oils
Zearalenone Immunoaffinity Purification Kit utilizes advanced magnetic beads technology for efficient extraction and purification of ZEN mycotoxin from various sample matrices.
Zearalenone (ZEN), also known as F-2 toxin, is a mycotoxin mainly produced by Fusarium species. It primarily contaminates cereals such as corn, wheat, rice, barley, millet, and oats. Zearalenone is heat-stable and can only be completely destroyed after treatment at 110°C for 1 hour.
Chemically, zearalenone is a phenolic lactone derivative of dihydroxybenzoic acid with the molecular formula C₁₈H₂₂O₅. It is insoluble in water, carbon disulfide, and carbon tetrachloride, but soluble in alkaline aqueous solutions, ether, benzene, chloroform, dichloromethane, ethyl acetate, and acids, and slightly soluble in petroleum ether.
Zearalenone exhibits estrogenic activity, approximately one-tenth that of natural estrogen, and can elevate estrogen levels in poultry and livestock. Pigs are known to be particularly sensitive to this toxin. Zearalenone primarily affects the reproductive system of female animals, but it also exerts certain effects on males. In cases of acute poisoning, it can cause toxicity to the nervous system, heart, kidneys, liver, and lungs. Its main mechanism involves overstimulation of the nervous system and hemorrhagic lesions in internal organs, which may lead to sudden death in animals.
The purification principle of this Zearalenone Immunoaffinity Purification Kit is as follows: the zearalenone (ZEN) in the sample is first extracted into the supernatant using an extraction solution. Magnetic beads coated with ZEN-specific antibodies selectively adsorb ZEN from the supernatant. Under the influence of an external magnetic field, impurities are removed and the bound zearalenone is eluted.
Finally, the concentration of ZEN in the sample is determined using analytical methods such as high-performance liquid chromatography (HPLC), ultra-high-performance liquid chromatography (UHPLC), or liquid chromatography-mass spectrometry (LC-MS).
This Zearalenone Immunoaffinity Purification Kit is suitable for the purification of zearalenone (ZEN) in corn, wheat, brown rice, oils, and their derived products.
Ideal for: Food safety laboratories, quality control departments, agricultural testing facilities, and research institutions focusing on mycotoxin analysis.
| Parameter | Specification |
|---|---|
| Maximum Binding Capacity | 1500 ng |
| Recovery Rate | ≥ 85% |
| Method | Magnetic Beads Immunoaffinity |
| Detection Limit | Compatible with HPLC, UHPLC, LC-MS |
| Component | Quantity |
|---|---|
| Reagent Cartridge | 20 Tests |
| Instruction Manual | 1 Copy |
| Inspection Report | 1 Copy |
| Well Position | Component | Volume |
|---|---|---|
| 1 | Diluent | 9 mL |
| 2 | Magnetic Beads Solution | 1 mL |
| 3 | Washing Solution | 1 mL |
| 4 | Washing Solution | 1 mL |
| 5 | Elution Solution | 1 mL |
Operate at an ambient temperature between 20-27 °C for optimal performance of the Zearalenone Immunoaffinity Purification Kit.
The maximum purification capacity of the kit is 1500 ng. When the zearalenone concentration in the sample exceeds 3000 ppb, the sample should be diluted before extraction.
Once the reagent compartment is unsealed, use it as soon as possible to avoid reagent volatilization that may lead to inaccurate results.
Do not use any reagent compartment showing leakage or damage.
Store the Zearalenone Immunoaffinity Purification Kit at 2-8 °C, sealed and dry. Do not freeze. Avoid exposure to direct sunlight.
12 months under recommended storage conditions from the date of manufacture.
Note: For consistent performance, ensure the kit is stored properly and used before the expiration date.
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